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gapdh reference gene sequence  (OriGene)


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    OriGene gapdh reference gene sequence
    Gapdh Reference Gene Sequence, supplied by OriGene, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sequence+standard+reference/GAPDH+Human+qPCR+Template+Standard/pm40933471-98-1-8
    Average 93 stars, based on 5 article reviews
    gapdh reference gene sequence - by Bioz Stars, 2026-11
    93/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Receptor Variation and Susceptibility to Middle East Respiratory Syndrome Coronavirus Infection
    Article Snippet: .. Untagged mouse (Ms) DPP4 plasmid pCMV-Kan/Neo-msDPP4 (GenBank BC022183) and C-terminal Myc/FLAG-tagged human (Hu) DPP4 plasmid pCMV6Entry-huDPP4 (NCBI Reference Sequence NM_001935) were purchased from OriGene. pCMV-Kan/Neo-msDPP4 was PCR amplified with the following primers: AsiSI-forward (5=-TTGGCGATCGCCATGAAGACA CCGTGGAAG-3=) and NotI-reverse (5=-ATAGTTTAGCGGCCGCAGT GTAAGGAGAAGCACTGCT-3=). ..

    Article Title: Syndecan-4 proteoliposomes enhance fibroblast growth factor-2 (FGF-2)-induced proliferation, migration, and neovascularization of ischemic muscle
    Article Snippet: .. A constitutive expression vector containing the full-length syndecan-4 gene (Origene; National Center for Biotechnology Information Reference Sequence {"type":"entrez-nucleotide","attrs":{"text":"NM_002999.3","term_id":"318037226","term_text":"NM_002999.3"}} NM_002999.3 ) was transfected into HeLa cells using the FuGENE HD transfection reagent (Roche) per the manufacturer's specifications. .. Using blunt dissection, a pocket was created in the s.c. space, and a small osmotic pump (DURECT) was implanted containing 5 μg of FGF-2 in combination with various treatments.

    Sequencing:

    Article Title: Receptor Variation and Susceptibility to Middle East Respiratory Syndrome Coronavirus Infection
    Article Snippet: .. Untagged mouse (Ms) DPP4 plasmid pCMV-Kan/Neo-msDPP4 (GenBank BC022183) and C-terminal Myc/FLAG-tagged human (Hu) DPP4 plasmid pCMV6Entry-huDPP4 (NCBI Reference Sequence NM_001935) were purchased from OriGene. pCMV-Kan/Neo-msDPP4 was PCR amplified with the following primers: AsiSI-forward (5=-TTGGCGATCGCCATGAAGACA CCGTGGAAG-3=) and NotI-reverse (5=-ATAGTTTAGCGGCCGCAGT GTAAGGAGAAGCACTGCT-3=). ..

    Article Title: IRX2-mediated upregulation of MMP-9 and VEGF in a PI3K/AKT-dependent manner.
    Article Snippet: .. The coding sequence of human IRX2 (NM_033267.4; NCBI Reference Sequence, OriGene Technologies, Rockville, MD, USA) was amplified by performing polymerase chain reaction (PCR) using the forward pr imer, 5'-GGATCCATGTCCTACCCGCAGGGC-3', which introduced a BamHI site, and the reverse primer, 5'-GAATTCCTATAGGTAGGGCTGGACGC-3' (Sangon Biotech Co., Ltd., Shanghai, China), which introduced an EcoRI site. .. The PCR conditions were as follows: Initial denaturation at 98 ̊C for 2 min, followed by 30 cycles of denaturation at 98 ̊C for 15 sec, primer annealing at 55 ̊C for 15 sec, and a primer extension step at 72 ̊C for 2 min, using high‐fidelity polymerase PrimeStar (Takara Bio Inc., Otsu, Japan).

    Article Title: Human and Pneumococcal Cell Surface Glyceraldehyde-3-phosphate Dehydrogenase (GAPDH) Proteins Are Both Ligands of Human C1q Protein
    Article Snippet: .. The human GAPDH gene was amplified from cDNA (GAPDH, NCBI Reference Sequence {"type":"entrez-nucleotide","attrs":{"text":"NM_002046","term_id":"576583510","term_text":"NM_002046"}} NM_002046 , human cDNA clone, Origene), and a tobacco etch virus cleavage site was introduced at the 5′-extremity. .. The resulting PCR product was cloned into the pETDuet vector (Novagen) to fuse a His 6 tag at the N terminus of the human GAPDH sequence.

    Article Title: Identification of Diagnostic Biomarkers for Myocardial Infarction Using Bioinformatics and Disulfidptosis-Targeted Computational Drug Discovery.
    Article Snippet: .. The GAPDH reference gene sequence was obtained from OriGene (https://www.origene.com.cn/). ..

    Article Title: Syndecan-4 proteoliposomes enhance fibroblast growth factor-2 (FGF-2)-induced proliferation, migration, and neovascularization of ischemic muscle
    Article Snippet: .. A constitutive expression vector containing the full-length syndecan-4 gene (Origene; National Center for Biotechnology Information Reference Sequence {"type":"entrez-nucleotide","attrs":{"text":"NM_002999.3","term_id":"318037226","term_text":"NM_002999.3"}} NM_002999.3 ) was transfected into HeLa cells using the FuGENE HD transfection reagent (Roche) per the manufacturer's specifications. .. Using blunt dissection, a pocket was created in the s.c. space, and a small osmotic pump (DURECT) was implanted containing 5 μg of FGF-2 in combination with various treatments.

    Polymerase Chain Reaction:

    Article Title: Receptor Variation and Susceptibility to Middle East Respiratory Syndrome Coronavirus Infection
    Article Snippet: .. Untagged mouse (Ms) DPP4 plasmid pCMV-Kan/Neo-msDPP4 (GenBank BC022183) and C-terminal Myc/FLAG-tagged human (Hu) DPP4 plasmid pCMV6Entry-huDPP4 (NCBI Reference Sequence NM_001935) were purchased from OriGene. pCMV-Kan/Neo-msDPP4 was PCR amplified with the following primers: AsiSI-forward (5=-TTGGCGATCGCCATGAAGACA CCGTGGAAG-3=) and NotI-reverse (5=-ATAGTTTAGCGGCCGCAGT GTAAGGAGAAGCACTGCT-3=). ..

    Article Title: IRX2-mediated upregulation of MMP-9 and VEGF in a PI3K/AKT-dependent manner.
    Article Snippet: .. The coding sequence of human IRX2 (NM_033267.4; NCBI Reference Sequence, OriGene Technologies, Rockville, MD, USA) was amplified by performing polymerase chain reaction (PCR) using the forward pr imer, 5'-GGATCCATGTCCTACCCGCAGGGC-3', which introduced a BamHI site, and the reverse primer, 5'-GAATTCCTATAGGTAGGGCTGGACGC-3' (Sangon Biotech Co., Ltd., Shanghai, China), which introduced an EcoRI site. .. The PCR conditions were as follows: Initial denaturation at 98 ̊C for 2 min, followed by 30 cycles of denaturation at 98 ̊C for 15 sec, primer annealing at 55 ̊C for 15 sec, and a primer extension step at 72 ̊C for 2 min, using high‐fidelity polymerase PrimeStar (Takara Bio Inc., Otsu, Japan).

    Amplification:

    Article Title: Receptor Variation and Susceptibility to Middle East Respiratory Syndrome Coronavirus Infection
    Article Snippet: .. Untagged mouse (Ms) DPP4 plasmid pCMV-Kan/Neo-msDPP4 (GenBank BC022183) and C-terminal Myc/FLAG-tagged human (Hu) DPP4 plasmid pCMV6Entry-huDPP4 (NCBI Reference Sequence NM_001935) were purchased from OriGene. pCMV-Kan/Neo-msDPP4 was PCR amplified with the following primers: AsiSI-forward (5=-TTGGCGATCGCCATGAAGACA CCGTGGAAG-3=) and NotI-reverse (5=-ATAGTTTAGCGGCCGCAGT GTAAGGAGAAGCACTGCT-3=). ..

    Article Title: IRX2-mediated upregulation of MMP-9 and VEGF in a PI3K/AKT-dependent manner.
    Article Snippet: .. The coding sequence of human IRX2 (NM_033267.4; NCBI Reference Sequence, OriGene Technologies, Rockville, MD, USA) was amplified by performing polymerase chain reaction (PCR) using the forward pr imer, 5'-GGATCCATGTCCTACCCGCAGGGC-3', which introduced a BamHI site, and the reverse primer, 5'-GAATTCCTATAGGTAGGGCTGGACGC-3' (Sangon Biotech Co., Ltd., Shanghai, China), which introduced an EcoRI site. .. The PCR conditions were as follows: Initial denaturation at 98 ̊C for 2 min, followed by 30 cycles of denaturation at 98 ̊C for 15 sec, primer annealing at 55 ̊C for 15 sec, and a primer extension step at 72 ̊C for 2 min, using high‐fidelity polymerase PrimeStar (Takara Bio Inc., Otsu, Japan).

    Article Title: Human and Pneumococcal Cell Surface Glyceraldehyde-3-phosphate Dehydrogenase (GAPDH) Proteins Are Both Ligands of Human C1q Protein
    Article Snippet: .. The human GAPDH gene was amplified from cDNA (GAPDH, NCBI Reference Sequence {"type":"entrez-nucleotide","attrs":{"text":"NM_002046","term_id":"576583510","term_text":"NM_002046"}} NM_002046 , human cDNA clone, Origene), and a tobacco etch virus cleavage site was introduced at the 5′-extremity. .. The resulting PCR product was cloned into the pETDuet vector (Novagen) to fuse a His 6 tag at the N terminus of the human GAPDH sequence.

    Virus:

    Article Title: Human and Pneumococcal Cell Surface Glyceraldehyde-3-phosphate Dehydrogenase (GAPDH) Proteins Are Both Ligands of Human C1q Protein
    Article Snippet: .. The human GAPDH gene was amplified from cDNA (GAPDH, NCBI Reference Sequence {"type":"entrez-nucleotide","attrs":{"text":"NM_002046","term_id":"576583510","term_text":"NM_002046"}} NM_002046 , human cDNA clone, Origene), and a tobacco etch virus cleavage site was introduced at the 5′-extremity. .. The resulting PCR product was cloned into the pETDuet vector (Novagen) to fuse a His 6 tag at the N terminus of the human GAPDH sequence.

    Expressing:

    Article Title: Syndecan-4 proteoliposomes enhance fibroblast growth factor-2 (FGF-2)-induced proliferation, migration, and neovascularization of ischemic muscle
    Article Snippet: .. A constitutive expression vector containing the full-length syndecan-4 gene (Origene; National Center for Biotechnology Information Reference Sequence {"type":"entrez-nucleotide","attrs":{"text":"NM_002999.3","term_id":"318037226","term_text":"NM_002999.3"}} NM_002999.3 ) was transfected into HeLa cells using the FuGENE HD transfection reagent (Roche) per the manufacturer's specifications. .. Using blunt dissection, a pocket was created in the s.c. space, and a small osmotic pump (DURECT) was implanted containing 5 μg of FGF-2 in combination with various treatments.

    Transfection:

    Article Title: Syndecan-4 proteoliposomes enhance fibroblast growth factor-2 (FGF-2)-induced proliferation, migration, and neovascularization of ischemic muscle
    Article Snippet: .. A constitutive expression vector containing the full-length syndecan-4 gene (Origene; National Center for Biotechnology Information Reference Sequence {"type":"entrez-nucleotide","attrs":{"text":"NM_002999.3","term_id":"318037226","term_text":"NM_002999.3"}} NM_002999.3 ) was transfected into HeLa cells using the FuGENE HD transfection reagent (Roche) per the manufacturer's specifications. .. Using blunt dissection, a pocket was created in the s.c. space, and a small osmotic pump (DURECT) was implanted containing 5 μg of FGF-2 in combination with various treatments.



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